ABSTRACT
COVID-19 has become one of the most significant events in the history of globalization. The prolonged 'lockdown' adopted across various countries in the world as a countermeasure for containing the spread of the virus profoundly brought forth socio-economic and infrastructural vulnerabilities in urban as well as rural parts of India. While urban and rural areas have been greatly studied with respect to the environment, human health, safety, livelihoods, associated risks, etc., in the context of pandemics, many of these studies seldom accommodate their interdependency as a pragmatic approach to planning. This is observed to be primarily due to the dynamic and diverse nature of interactions coupled with the development disparities between rural and urban areas, thereby adding complexity to development decision making. The present study, therefore, applies the lens of the circulating and ecological sphere (CES), introduced by the Japanese government for the localization of resource flows between urban–rural regions, to consider possible alternative development approaches to achieve smooth transitions during pandemics through the case study area located in Hingna tahsil in the Nagpur Metropolitan Area, India. The methodology uses the critical examination of rural–urban linkages amidst the crisis through key-informant surveys involving representatives from local governments. Using this feedback and spatial analysis tools, the research identifies probable entry points in post-pandemic regional planning. The research contributes to understanding the impact of spatial development during pandemics through ground-based evidence. The findings from this research highlight the need to manage rural dependencies on urban areas and underline the potential of the rural–urban linkage as an approach, acknowledged and emphasized through CES, for managing such regional-scale hazards. The investigation concludes with the discussion and future research scope for achieving the pronounced needs reflected through the study.
ABSTRACT
Rapid nucleic-acid based tests that can be performed by non-professionals outside laboratory settings could help the containment of the pandemic SARS-CoV-2 virus and may potentially prevent further widespread lockdowns. Here, we present a novel compact portable detection instrument (the Egoo Health System) for extraction-free detection of SARS-CoV-2 using isothermal reverse transcription strand invasion based amplification (RT-SIBA). The SARS-CoV-2 RT-SIBA assay can be performed directly on crude oropharyngeal swabs without nucleic acid extraction with a reaction time of 30 min. The Egoo Health system uses a capsule system, which is automatically sealed tight in the Egoo instrument after applying the sample, resulting in a closed system optimal for molecular isothermal amplification. The performance of the Egoo Health System is comparable to the PCR instrument with an analytical sensitivity of 25 viral RNA copies per SARS-CoV-2 RT-SIBA reaction and a clinical sensitivity and specificity between 87.0-98.4% and 96.6-98.2% respectively.